surface topography and thickness of hybrid layer after pretreatment of dentin surface with egg shell powder as desensitizing agent Ramy Abdallah1, Hadeel Farouk2, Mona E Essa3 1 lecturer of dental material, Faculty of Dentistry for boys, Alazhar University, Egypt.
2 Lecturer of Conservative Dentistry, Faculty of oral and dental medicine, Ahram Canadian University, Egypt. 3 Associate Professor of Operative Dentistry, Faculty of Dentistry, Beni-Suef University, Egypt. Abstract Objectives: The aim is to investigate the effect of Eggshell solution (ESS) desensitizing agents as dentin surface pretreatments on thickness of hybrid layer of a direct resin composite restoration bonded with etch and rinse adhesive systems. Methods: A flat dentin occlusal surface will be prepared on extracted molar using a low speed diamond saw under water coolant. Teeth will be randomly divided into three main groups according to dentin surface pretreatment.
First group, no dentin pretreatment (A1), second group Egg shell pretreatment applied before acid etching (A2) and third group, Egg shell pretreatment applied after acid etching (A3). A Teflon mold will be used to build up resin composite directly. Teeth will be sectioned perpendicular to adhesive joint to obtain 0.9±0.1 mm in thickness slabs. All samples will be examined under scanning electron microscope (SEM) and thickness of hybrid layer will be measured. Moreover energy dispersive analytical x-ray (EDAX) analysis will be used to count different minerals at tooth restoration interface. Results: The SEM analysis showed that; there are established significant differences in the thickness of hybrid layers among all tested groups.
The EDAX analysis showed increased quantitative amounts of Ca atomic % and P atomic % for the ESS treated groups. Conclusion: Eggshell solution when used as desensitizing agent can significantly increase the hybrid layer thickness. Key words: Eggshell powder, Dentin, Hybrid layer thickness, Desensitizing agent. 1- Introduction Dentinal tubules play a major role in transferring stimuli and irritants to the pulp (1).
One of the most common cause of postoperative dentin hypersensitivity (DH) of tooth that restored with resin-composite which pretreated with the etch-and-rinse adhesive system, the higher dentinal permeability of dentin surface; in combination with, the incomplete sealing of the opened dentinal tubules, so; the dentinal fluid transudation with the un-polymerized adhesive may lead to formation of blisters along the adhesive-tooth interface that filled with water (2-4). The hydrodynamic theory that considered the most accepted theory that can explain the dentin hypersensitivity(1,5) was used to illustrate the effect of water-filled blisters as follow; during mastication; these blisters may compressed causing higher rate of dentinal fluid movement within the dentinal tubules indirectly stimulating the extremities of the pulp nerves causing the postoperative dentinal hypersensitivity(4). Dentinal hypersensitivity (DH) can be defined as “the short exaggerated, painful response elicited when exposed dentin is subjected to certain thermal, mechanical or chemical stimuli (6). The treatment and prevention of DH using the concept of dentin desensitization through the mechanical occlusion approach of the dentinal tubules by using chemical or physical occluding agent that can reduce the DH through reducing the dentin permeability and the movement of pulpal fluid(7, 8). The Eggshell powder (ES) and Nano-hydroxyapatite (Nano-HA) containing dentine like substance are examples to the physically approach which plugs the open dentinal tubules with a calcium and phosphate that precipitated and prevent the fluid diffusion through the tubules into the dentin sub-surface (9,10). Eggshell is a rich source of minerals, mainly calcium carbonate and is probably the best natural source of calcium (11).
The eggshell contains mainly calcium, phosphorous, magnesium, strontium, and fluoride (9,12). Demineralization resulting from loss of calcium and phosphate ions can be restored by using non-invasive calcium phosphate delivery system (12,13), like eggshell solution(14). Dentinal hybrid layer (HL) is a mixture of polymerized resin infiltrated in partially demineralized dentin at a molecular-level (15). Both of smear layer and the mineralized dentin don’t allow monomer diffusion into the collagen network (16). Therefore, dentine “conditioning” must be done to remove or modify of the smear layer and to demineralize the mineralized surface dentin, thus permitting monomer diffusion into the demineralized collagen network and formation of hybrid layer(15,16). The etch-and-rinse approach (gold standard approach) can readily be recognized by an initial etching step, the so-called “conditioning step”, followed by a compulsory rinsing phase(17).
After the conditioning step, adhesion-promoting monomers are applied in one or two application steps to penetrate the exposed collagen network (17,18). The process of dentin hybridization completely changes the physical and chemical properties of dentin and hence can help in dentin desensitization (12,15). 2- Material and Methods Materials which are used in the present study have been illustrated in Table (1). (Table 1) Materials, Chemical Composition and Manufacture Material Chemical Composition Manufacture Chicken Egg Shell Powder – Egg shell powder Prepared at Faculty of Science, Tanta University Scotch bond universal Etchant – 34% phosphoric acid with a pH approximately 0.1. 3M ESPE, USA.
Scotch bond universal adhesive – Single component light curing adhesive. – 10-methacryloxydecyl dihydrogen phosphate (MDP) and silane. – Ethanol-and water based adhesive. Bisco, Inc, USA. 3M Z 350 Nano filled resin composite BIS-GMA, BIS-EMA, UDMA, TEGDMA resin.
Non-agglomerated/non-aggregated 20 nm silica filler. non-agglomerated / non-aggregated 4 to 11 nm zirconia filler, and aggregated zirconia/silica cluster filler (comprised of 20 nm silica and 4 to 11 nm zirconia particles). Shade A3E. 3M ESPE, USA. Egg Shell powder Preparation 1- Calcination Process This process used to prepare the calcium oxide from chicken egg shell (CES), the egg shell cleaned in tap water firstly, then kept in a hot water bath at 100°C for 10 minutes to facilitate removal of the internal protein membrane from the shell.
After that; the egg shell was crashed using a sterile mortar and pestle and heated to in a hot air oven at 1100C for 12hrs (14) by furnace (Faculty of Science, Tanta University), at this temperature the shell becomes porous, fragile and very white in color. It is concluded from this fact that the egg shell CaCO3 decomposes (decarbonation process) and gave CaO and CO2, according to the reaction: ? CaCO3 ? CaO +CO2 (14, 19). 2- Preparation of Egg Shell Desensitizing Solution ?? To prepare the egg shell desensitizing solution; one gram of the previously prepared egg shell powder was dissolved in 20 ml of 4% acetic acid in a test tube. The clear fluid which is collected at the top was then transferred to a beaker and the pH of the solution was tested using a pH meter (JENWAY, 3505, Keison, UK) which was 11.7(14,20).
Sample Preparation Thirty molar teeth were selected for this study. All collected teeth were extracted for therapeutic reasons from patients of age group (35-45 years). The selected teeth were free of caries, cracks and showed no apparent hypoplastic defects. The selected teeth were thoroughly cleaned from calculus, tissue deposits, polished with pumice and rotating brush at conventional speed.
The teeth were stored in saline solution at room temperature until the time of their use reference. The occlusal enamel of teeth were removed perpendicular to the long axis of teeth, to expose flat dentin surface at a standardized depth. Then a line was drawn 2mm below DEJ using a caliper. The teeth were cut horizontally and flattened under copious water coolant.
The occlusal tables were ground with a rotary grinding milling machine using #180-grit silicon carbide papers under continuous water coolant to create a uniform thickness of smear layer (4,21). The teeth were divided into three main groups of ten teeth each; according to the surface pretreatment done namely; first group, no dentin pretreatment (A1), second group, Egg shell pretreatment applied before acid etching (A2) and third group, Egg shell pretreatment applied after acid etching (A3). Etch and rinse adhesive bonding The flat dentin occlusal surfaces were etched (conditioning) using Scotch bond universal Etchant for 15 seconds, rinsed for 10 seconds, and blotted dry with absorbent sponge pellet leaving the dentin surface visibly moist. Two consecutive coats of adhesive system (Scotch bond Universal adhesive) were applied (Group A1) using a fully saturated brush tip and gently air-thinned for 5 seconds leaving a shiny surface and then polymerized with a light-emitting diode (LED) light curing unit (Elipar LED Curing Light; 3M ESPE) for 20 seconds according to the manufacturer’s instructions(21).
Egg shell powder solution (ESS) then passively applied on un-etched dentin using a brush for 5 minutes (9,10) (Group A2). The dentin was then rinsed for 5 seconds then dried gently with absorbent paper. While (Group A3); the ES solution applied to the etched dentin. Resin Composite Restorative Material Application A specially constructed two halves spilt Teflon round mold with a central square hole (5 mm X 5 mm in diameter and 4 mm in depth) was fabricated for resin composite build up.
Nano filled visible light resin composite (Filtek Z350, 3M ESPE, USA), was built up into two increments each 2mm in thickness on dentin wall. Each increment was packed using Teflon tipped instrument then light cured for 20 seconds using light-emitting diode Curing Light (Elipar LED Curing Light; 3M ESPE). Last increment was cured against celluloid strip matrix to avoid oxygen inhibited layer. Each tooth was mounted on the cutting machine (Bronwill, E.
McGrath Inc), and sectioned into a series of 1 mm thick slabs under water coolant. The sectioning was performed using a diamond disc of 0.3 mm thickness (IPDB40305, MTI Corporation, Richmond, USA) (10,21). ten Slabs from each group were examined using scanning electron microscope. The tooth restoration interface of each slab were air dried, mounted on aluminium stubs and sputter-coated with gold for 2 minutes and examined with a scanning electron microscope (SEM) (LEO Electron Microscopy Ltd., Cambridge, UK) operating at 10-20 kV and 4000X magnification. Thickness of hybrid layer was measured at 5 points on each slab (10,21).
Using the EDAX (energy dispersive analytical x-ray), the amount of Ca and P ions within the adhesive layer, hybrid layer and resin tags, in each specimen was measured in an area of (120µm X 120 µm) at 240000x magnification directly on the SEM microscope monitor (10). The data were analyzed by SPSS version 20 using One-ANOVA. The test was performed to determine a statistically significant difference in hybrid layer thickness, and Tukey’s post hoc multiple comparisons test was used to compare between more than two groups in non-related samples, test and P ? 0.05 was considered statistically significant. 3- Results SEM Analysis Results of the SEM analysis showed that; there are established significant differences in the thickness of hybrid layers among all tested groups.
The SEM analysis showed that the highest mean value of hybrid layer thickness was found in (Group A3) (35.86 ?m) followed by (Group A2) (22.26 ?m), and the lowest mean value of hybrid layer thickness was found in (Group A1) (14.04 ?m) (Table 2 and Figure 1). Multiple group comparison showed that there was significantly different among the all tested groups. SEM image of the tested groups (Group A1 and Group A2) showed a continuous and homogenous hybrid layer thickness distribution without any gap formation at the dentin–adhesive interface at the scanned area. In addition, SEM image of resin/dentin interface for (Group A3) showed a gap formation and the dentinal tubules blocked with egg-shell minerals.
Table (2): The mean, standard deviation (SD) of thickness of hybrid layer thickness in all tested groups. Variables Thickness of hybrid layer Mean SD Group (A1) 14.04 c 0.91 Group (A2) 22.26 b 0.68 Group (A3) 35.86 a 0.73 p-value ;0.001* Means with different letters in the same column indicate statistically significance difference. Figure 1: SEM image showed the hybrid layer thickness among all tested groups. Group A1 Group A2 Group A3 Atomic Analysis by EDX The results of atomic analysis of calcium and phosphorus in all samples by energy dispersive X-ray spectrometry showed that; although there is no significant difference in Ca/P ratio among the (Group A1) and (Group A2), but the quantitative amounts of Ca atomic % and P atomic % is greater for the ES treated groups (Group A2 and A3), while the untreated group, demineralized group, (Group A1) shows the lowest Ca and P atomic % (Table 3).
Table (3): Ca / P atomic ratio of all tested groups. Group Ca% P% Ca/P atomic ratio Group (A1) 5.95 5.66 1.05 Group (A2) 8.84 8.4 1.05 Group (A3) 18.93 11.47 1.65 4- Discussion Dentin is the core hard substance of the tooth which is covered by enamel on the crown and cementum on the root. Dentin is the calcified product of the odontoblasts which line the inner surface of the dentin within the periphery of the external pulp tissue. Therefore, the dentin and pulp are morphologically and embryologically a single unit (22). Hybridized dentin reduces the risk of microleakage, the incidence of secondary caries and post-operative sensitivity that can be caused by such leakage (23).
Also, hybrid layer and resin tags formation have been reported to play a major role in resin retention (23). Coronal dentin discs were used in this study to evaluate tubule occlusion and depth of penetration of the desensitizing agents into the tubules. Important variables such as dentin surface area, thickness, and surface characteristics can be controlled in coronal dentin discs compared to cervical dentin discs. This model was found appropriate for application of the experimental agents on a flat dentin surface and also enabled standardized comparisons of different treatment protocols with ease.
This remains a time-tested screening model to study tubule occlusion by potential desensitizing agents (24). Since HAp exhibits excellent bioactive properties, this may be considered the material of choice for treating hypersensitivity in the near future (25). Although various sources are available for the synthesis of HAp, chicken eggshells were chosen in this study owing to its high calcium content and cost effectiveness. These eggshell waste help in reducing the cost of high-quality calcium source and at the same time promote recycle of material (26,27). The process of egg shell calcination is responsible for pathogens removal and an increase in its alkalinity (28,20).
Also, the calcination process is responsible for calcium carbonate formation which acts as a calcium rich layer that can binds to the negatively charged dentine surface resulting in the plugging of dentine tubules and blocking diffusion of fluids and acts as desensitizing agent (10). In the current study, the thickness of the hybrid layer formed was studied using an SEM. However, SEM has certain benefits for analyzing surfaces and interfaces(29) and has become the most popular and easiest tool to morphologically examine the bonding mechanism and bonding interfaces. Also, a few SEM studies indicating the effective thickness of hybrid layer have been published (16).
In etch-and-rinse systems, phosphoric acid treatment not only removes possible superficial debris, the so-called smear layer, but also exposes a net of collagen fibers besides opening the dentin tubules. By penetrating this collagen mesh and dentin tubules, the infiltrating resin will form two well-defined structures which are known as hybrid layer and resin tags, respectively(30,17). Collagen fiber network should be in a fully expanded state to facilitate resin penetration “wet bonding” (30,17). The effective bonding relies mostly on the ability of the bonding agent to completely infiltrate the exposed collagen mesh, ideally sealing and protecting it from all sorts of degradation pathways; i.e. quality of hybrid layer.
In the same way, it has been suggested that the bonding effectiveness does not depend on the number nor on the length of resin tags (31,32). In (Group A1); when using etch-and-rinse systems, proper hybrid layer is classically achieved through the infiltration of resin monomers into the exposed collagen mesh by using the so-called wet- bonding technique. In this protocol, water remaining from the rinsing step maintains the collagen network expanded, allowing resin monomers to properly infiltrate it and hybrid layer formation (30,33). The hybrid layer of (Group A2) was thicker when compared to (Group A1); may depend on effective penetration of bonding adhesive (MDP) to partially demineralized dentin (hybrid layer formation) (34), as the application of eggshell solution on dentin surface before acid etching may lead to decreasing the demineralizing action of acid etch due to the buffering capacity of the eggshell minerals and its high alkalinity. So, after rinsing, collagen fibrils are not completely deprived from hydroxyapatite, leaving residual hydroxyapatite still attached to collagen, which may serve as a receptor for additional chemical bonding(35), that emphasis by our EDX results which showed in (Table 3).
The application of carboxylic acid-based monomers “functional monomer”, 10-methacryloxydecyl dihydrogen phosphate (MDP), which included in the bonding agent(36), have a chemical bonding potential to calcium of residual hydroxyapatite(35). So, the increased thickness of hybrid layer may be due to the possibility of incorporating the residual minerals into the hybrid layer and formation of “hybridized complex” which comprises two portions: the zone of authentic hybrid layer and the zone of hybridized minerals (37,38). So, the addition of eggshell solution before etching have benefit of formation of thick hybrid layer in addition to desensitization of dentin by residual hydroxyapatite(39,40). The thickness of hybrid layer of (Group A3) showed the maximum value when compared to (Group A1) and (Group A2), which were statistically significant. Possibly could be due to that in case of (Group A3), the specimens were etched with phosphoric acid and the reaction products were rinsed off. This results in complete removal of smear layer and smear plugs from the dentin, so that the dentin permeability increases (2) then, after the application of eggshell solution on etched dentin surface, calcium plays an active role due to a very high percentage of bio-available calcium in ES solution(14,41).
Also, the pH of a ESP solution was 11.7, which is favorable, as it increases the ion activity of anions such as hydroxyl ions in the solution (14,41). The water which retained in collagen fibrils and in dentinal tubules act as a vehicle and facilitate the movement of calcium ion through it(3), then 10-MDP (10-methacry- loxydecyl dihydrogen phosphate)(36) present in universal adhesives have a chemical bonding potential to calcium ions in both collagen fibrils and in dentinal tubules forming a thicker hybrid layer. In (group A3); when the water inside the collagen network is not completely displaced, the polymerization of resin inside the hybrid layer may be affected or least, the remaining water will compete for space with resin inside the demineralized dentin (42). That results in gap formation as showed in (Figure 1). 5- Conclusion Under the limitations of this in vitro study, it can be concluded that all the ES solution as experimental agents can occluded the dentinal tubules to varying degrees and also penetrated to varying depths into the dentinal tubules forming different thickness of hybrid layer depending on its application strategy (before or after dentin etching).
Although dense hybrid layer and occluded dentinal tubules combination was effective to desensitize the dentin and prevent the post-operative hypersensitivity, further clinical studies are needed to determine its effectiveness over time. 6-Recomentation The availability of using egg shell powder clinically as desensitizing agent. 7- References 1- Braennstroem M, Astroem A. A study on the mechanism of pain elicited from the dentin. Dent Res.
1964;43:619-25.? 2- Tay FR, Gwinnett AJ, Wei SH. The over wet phenomenon: a transmission electron microscopic study of surface moisture in the acid-conditioned, resin-dentine interface. Am J Dent. 1996; 9:161–6.
3- Tay FR, Pashley DH, Mak YF, Carvalho RM, Lai SC, Suh BI. Integrating oxalate desensitizers with total-etch two-step adhesive. J Dent Res. 2003; 82:703-7.
? 4- Awang RAR, Masudi SM, Mohd Nor WZW. Effect of desensitizing agent on shear bond strength of an adhesive system. Arch Orofac Sci. 2007; 2:32–5.
? 5- Sauro S, Gandol MG, Prati C, Mongiorgi R. Oxalate-containing phytocomplexes as dentine desensitizers: an in vitro study. Arch Oral Biol. 2006; 51:655–64.
? 6- Santiago SL, Pereira JC, Martineli AC. Effect of commercially available and experimental potassium oxalate-based dentin desensitizing agents in dentin permeability: Influence of time and ltration system. Braz Dent J. 2006;17:300- 05.
7- Walters PA. Dentinal hypersensitivity: a review. J Contemp Dent Pract. 2005; 6:107–17.
? 8- Rees JS. The prevalence of dentine hypersensitivity in general dental practice in the UK. J Clin Periodontol. 2000; 27:860–5.
9- Haghgoo R, Mehran M, Ahmadvand M, Ahmadvand MJ. Remineralization Effect of Eggshell versus Nano-hydroxyapatite on Caries-like Lesions in Permanent Teeth (In Vitro). J Inter Ora Heal. 2016; 8(4):435-9. 10- Kulal R, Jayanti I, Sambashivaiah S, Bilchodmath S. An In-vitro Comparison of Nano Hydroxyapatite, Novamin and Proargin Desensitizing Toothpastes – A SEM Study.
J Clini Diagno Res. 2016; 10(10): ZC51-ZC4. 11- King Ori A.M. A review of the uses of poultry eggshell and shell membrane. Int J poult sci.
2011; 10(11):908–12. 12- Kato MT LM, Sales-Pares SH, Buzalaf MA. Preventive effect of commercial desensitizing tooth pastes on bovine enamel erosion in vitro”. Caries Res. 2010; 44: 85-9.
13- Yaberi M, Haghgoo R. A comparative study of the effect of nanohydroxyapatite and eggshell on erosive lesions of the enamel of permanent teeth following soft drink exposure: A randomized clinical trial. J Int Oral Health 2018;10:176-9. 14- Feroz S, Moeen F, Nisar Haq S.?Protective Effect of Chicken Egg Shell Powder Solution (CESP) on Artificially Induced Dental Erosion: An in Vitro Atomic Force Microscope Study. IJDSR. 2017; 5(3): 49-55.
15- Santini A, Milia E, Miletic V. A review of SEM and TEM studies on the hybridisation of dentine. Microscopy: Science, Technology, Applications and Education; FORMATEX 2010; 256-68. 16- Tay F, Pashley D. Aggressiveness of contemporary self-etching systems.
I: Depth of penetration beyond dentin smear layers. Dent Mater 2001; 17:296–308.? 17- Kenshima S, Francci C, Reis A, Dourado Loguercio A, Rodrigues Filho L.E. Conditioning effect on dentin, resin tags and hybrid layer of different acidity self-etch adhesives applied to?thick and thin smear layer. JJOD 2006; 994: 1-9. 18- Carvalho R, Mendonca J, Santiago S, Silveira R, Garcia F, Tay Fetal.
Effects of HEMA/solvent combinations on bond strength to dentin. J Dent Res. 2003; 82:597–601. 19- Engin B, Demirtas H, Eken M.
Temperature effects on egg shells investigated by XRD, IR and ESR techniques J Radiat. Phys. ?Chem. 2006;75: 268–277.
? 20- Shen P MD, Cochrane NJ,Walker GD,Yuan Y, Reynolds C, et al. “Effect of added calcium phosphate on enamel remineralization by fluoride in a randomized controlled in situ trial”. J Dent. 2011; 39: 518-25. 21- Kumar AJ, Yogesh BG, Divakar DD, Dalati MHN, Felemban B, Alshadidi MYM.
Scanning Electron Microscopic Insight to the Hybrid Layer in Resin Composite Restored Cervical Lesions. J Inter Ora Heal. 2016; 8(4):415-2. 22- Nikaido T , Takahashi R, Ariyoshi M, Sadr A, Tagami J.
Protection and Reinforcement of Tooth Structures by Dental Coating Materials. Coatings 2012; 2: 210-20. 23- Kallepalli S, Dash S, Gopa S, Surya Kumari NBP. Evaluation of thickness of hybrid layer and length of resin tags of three adhesives to the root canal dentin: An in vitro scanning electron microscopic study. J Dr. NTR Univers of Heal Scienc 2014; 3(S1) ; S17-S22.
24- Kunam D, Manimaran S, Sampath V, Sekar M. Evaluation of dentinal tubule occlusion and depth of penetration of nano-hydroxyapatite derived from chicken eggshell powder with and without addition of sodium fluoride: An in vitro study. J Conser Dent. 2016; 19 (3): 239-44. 25- Watanabe J, Akashi M. Formation of hydroxyapatite provides a tunable protein reservoir within porous polyester membranes by an improved soaking process.
Biomacromolecules 2007;8:2288-93. 26- Sanosh KP, Chu MC, Balakrishnan A, Kim TN, Cho SJ. Utilization of biowaste eggshells to synthesize nanocrystalline hydroxyapatite powders. Mater Lett. 2009;63:2100-2.
27- Sasikumar S, Vijayaraghavan R. Low temperature synthesis of nanocrystalline hydroxyapatite from egg shells by combustion method. ?Trends Biomater Artif Orga. 2006;19:70-3.
? 28- Han Y, Xu K, Montay G, Fu T, Lu J. Evaluation of nanostructured carbonated hydroxyapatite coatings formed by a hybrid process of plasma spraying and hydrothermal synthesis. J Biomed Mater Res. 2002;60:511-6. 29- Yoshiyama M, Sano H, Ebisu S, Tagami J, Ciucchi B, Carvalho RM, et al. Regional strengths of bonding agents to cervical sclerotic root dentin.
J Dent Res. 1996;75(6):1404-13. ? 30- Mohan B, Kandaswamy D. A confocal microscopic evaluation of resin- dentin interface using adhesive systems with three different solvents bonded to dry and moist dentinan in vitro study. Quintessence Int 2005;36:511-21. 31- Yoshiyama M, Carvalho R, Sano H, Horner J, Brewer PD, Pashley DH.
Interfacial morphology and strength of bonds made to superficial versus deep dentin. Am J Dent. 1995;8(6):297-302. 32- Vallabhdas AK, kumar CNV, Kabbinale P, Nayak R, Rajakumari M, Shilpa T. Evaluation of hybrid layer and bonding interface after water storage with and without the usage of 2% chlorhexidine: A scanning electron microscope study.
J Contemp Dent Pract. 2018;19(1):52-59. 33- Pashley DH, Tay FR, Carvalho RM, Rueggeberg FA, Agee KA, Carrilho M et al. From dry bonding to water-wet bonding to ethanol-wet bonding: a review of the interactions between dentin matrix and solvated resins using a macromodel of the hybrid layer.
Am J Dent. 2007;20(1):7-20. 34- Nakabayashi N, Pashley DH. Hybridization of Dental Hard Tissues.
Chicago: Quintessence; 1998. 35- Yoshida Y, Nagakane K, Fukuda R, Nakayama Y, Okazaki M, tani H, Inoue S, et al. Comparative study on adhesive performan functional monomers. J Dent Res. 2004; 83(6): 454–8. ? 36- Li N, Nikaido T, Takagaki T, Sadr A, Makishi P, Chen J, Tagami J.
The role of functional monomers in bonding to enamel: Acid-base resistant zone and bonding performance. J Dent. 2010; 38, 722–30. 37- Waidyasekera K, Nikaido T, Weerasinghe DS, Ichinose S, Tagami J. Reinforcement of dentin in self-etch adhesive technology: A new concept.
J Dent. 2009; 37, 604–9. 38- Tay FR, Sano H, Carvalho RM. An ultrastructural study of the influence of acidity of self-etching primers and smear layer thickness on bonding to intact dentin. J Adhes Dent.
2000;2:83-98. 39- Torii Y, Itou K, Nishitani Y, Ishikawa K, Suzuki K. Effect of phosphoric acid etching prior to self-etching primer application on adhesion of resin composite to enamel and dentin. Am J Dent. 2002;15(5):305-8. 40- Marchesi G, Frassetto A, Mazzoni A, Apolonio F, Diolosà M, Cadenaro M, et al.
Adhesive performance of a multi- mode adhesive system: 1-year in vitro study. J Dent. 2014;42(5):603-12. 41- Mony B, Ebenezar R, Ghani M F, Narayanan A, Anand S, Mohan AG. Effect of Chicken Egg Shell Powder Solution on Early Enamel Carious Lesions: An Invitro Preliminary Study.
J Clinic Diagn Res. 2015; 9(3): ZC30-ZC32. 42- Jacobsen T, Söderhold KJ. Some effects of water on dentin bonding.
Dent Mater. 1995; 11(2): 132–6. ?